Journal: Renal Failure

Article Title: Inhibition of caspase-1 by ginsenoside Rg1 ameliorates d -gal-induced renal aging and injury through suppression of oxidative stress and inflammation

doi: 10.1080/0886022X.2025.2504634

Figure Lengend Snippet: Rg1 Delays renal senescence by inhibiting caspase-1. (A) The serum level of β-galactosidase (β-gal, mU) measured by ELISA kit. (B) RT-qPCR analysis of p53 transcription in kidney each group. (C) RT-qPCR analysis of p21 transcription in kidney in each group. (D) Immunoblots of p53 and p21 in kidney. (E) Relative protein expression of p53 in kidney. (F) Relative protein expression of p21 in kidney. The data are expressed as the means ± SEM, n = 3. * p < 0.05, ** p < 0.01, ** p < 0.001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) Kits utilized in the experiment are as follows: mouse serum creatinine (Scr) ELISA kit (mouse, JONLNBIO, Jiangsu, China, Cat# JL20633, RRID: AB_3661983); mouse Urea Nitrogen (BUN) ELISA Kit (mouse, Sbjbio, Nanjing, China, Cat# JBS-M0549, RRID: AB_3661991); mouse SOD ELISA KIT (mouse, YBIO, Shanghai, China, Cat# IC-SOD1-Mu, RRID: AB_3661984); mouse IL-18 ELISA KIT (mouse, Chuan Qiu Biotechnology, Shanghai, China, Cat# HM-022, RRID: AB_3661985); mouse IL-1 ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1516, RRID: AB_3661986); tumor necrosis factor-α (TNF-α) ELISA KIT (mouse, Bioss, Beijing, China, Cat# bsk12002, RRID: AB_3661987); mouse MDA ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1331, RRID: AB_3661988); mouse ROS ELISA KIT (mouse, Yan Yu Medical Reagent, Shanghai, China, Cat# YYu-ELISA-374950, RRID: AB_3661989); mouse beta galactosidases (β-GAL) ELISA KIT (mouse, KlBio, Shanghai, China, Cat# kl-M1264c, RRID: AB_3661990).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Expressing

Journal: Renal Failure

Article Title: Inhibition of caspase-1 by ginsenoside Rg1 ameliorates d -gal-induced renal aging and injury through suppression of oxidative stress and inflammation

doi: 10.1080/0886022X.2025.2504634

Figure Lengend Snippet: Rg1 Improves renal function injury by inhibiting caspase-1. (A) The serum level of blood urea nitrogen (BUN, pM) measured by ELISA kit. (B) The serum level of creatinine (scr, μM) measured by ELISA kit. (C) The results of HE staining of kidney tissue (HE staining 200 × and HE staining 400 ×). the data are expressed as the means ± SEM, n = 3. * p < 0.05, ** p < 0.01, ** p < 0.001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) Kits utilized in the experiment are as follows: mouse serum creatinine (Scr) ELISA kit (mouse, JONLNBIO, Jiangsu, China, Cat# JL20633, RRID: AB_3661983); mouse Urea Nitrogen (BUN) ELISA Kit (mouse, Sbjbio, Nanjing, China, Cat# JBS-M0549, RRID: AB_3661991); mouse SOD ELISA KIT (mouse, YBIO, Shanghai, China, Cat# IC-SOD1-Mu, RRID: AB_3661984); mouse IL-18 ELISA KIT (mouse, Chuan Qiu Biotechnology, Shanghai, China, Cat# HM-022, RRID: AB_3661985); mouse IL-1 ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1516, RRID: AB_3661986); tumor necrosis factor-α (TNF-α) ELISA KIT (mouse, Bioss, Beijing, China, Cat# bsk12002, RRID: AB_3661987); mouse MDA ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1331, RRID: AB_3661988); mouse ROS ELISA KIT (mouse, Yan Yu Medical Reagent, Shanghai, China, Cat# YYu-ELISA-374950, RRID: AB_3661989); mouse beta galactosidases (β-GAL) ELISA KIT (mouse, KlBio, Shanghai, China, Cat# kl-M1264c, RRID: AB_3661990).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Renal Failure

Article Title: Inhibition of caspase-1 by ginsenoside Rg1 ameliorates d -gal-induced renal aging and injury through suppression of oxidative stress and inflammation

doi: 10.1080/0886022X.2025.2504634

Figure Lengend Snippet: Rg1 improves renal inflammation and oxidative stress by inhibiting caspase-1. (A) The level of tumor necrosis factor-α (TNF-α, pg) in kidney measured by ELISA kit. (B)The level of malondialdehyde (MDA, nM) in kidney measured by ELISA kit. (C) The level of reactive oxygen species (ROS, U) in kidney measured by ELISA kit. (D) The level of superoxide dismutase (SOD, U) in kidney measured by ELISA kit. The data are expressed as the means ± SEM, n = 3. * p < 0.05, ** p < 0.01, ** p < 0.001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) Kits utilized in the experiment are as follows: mouse serum creatinine (Scr) ELISA kit (mouse, JONLNBIO, Jiangsu, China, Cat# JL20633, RRID: AB_3661983); mouse Urea Nitrogen (BUN) ELISA Kit (mouse, Sbjbio, Nanjing, China, Cat# JBS-M0549, RRID: AB_3661991); mouse SOD ELISA KIT (mouse, YBIO, Shanghai, China, Cat# IC-SOD1-Mu, RRID: AB_3661984); mouse IL-18 ELISA KIT (mouse, Chuan Qiu Biotechnology, Shanghai, China, Cat# HM-022, RRID: AB_3661985); mouse IL-1 ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1516, RRID: AB_3661986); tumor necrosis factor-α (TNF-α) ELISA KIT (mouse, Bioss, Beijing, China, Cat# bsk12002, RRID: AB_3661987); mouse MDA ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1331, RRID: AB_3661988); mouse ROS ELISA KIT (mouse, Yan Yu Medical Reagent, Shanghai, China, Cat# YYu-ELISA-374950, RRID: AB_3661989); mouse beta galactosidases (β-GAL) ELISA KIT (mouse, KlBio, Shanghai, China, Cat# kl-M1264c, RRID: AB_3661990).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Renal Failure

Article Title: Inhibition of caspase-1 by ginsenoside Rg1 ameliorates d -gal-induced renal aging and injury through suppression of oxidative stress and inflammation

doi: 10.1080/0886022X.2025.2504634

Figure Lengend Snippet: Changes of caspase-1 signal pathway in kidney tissue after Rg1 intervention. (A) qRT-PCR analysis of caspase-1 transcription in kidney in each group. (B) qRT-PCR analysis of IL-1 transcription in kidney in each group. (C) qRT-PCR analysis of IL-18 transcription in kidney in each group. (D) Western blots of caspase-1 of kidney tissue. (E) Relative protein expression of caspase-1 in kidney. (F) The level of interleukin-1 (IL-1, pg) in kidney measured by ELISA kit. (G) The level of interleukin-18 (IL-18, ng) in kidney measured by ELISA kit. The data are expressed as the means ± SEM, n = 3. * p < 0.05, ** p < 0.01, ** p < 0.001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) Kits utilized in the experiment are as follows: mouse serum creatinine (Scr) ELISA kit (mouse, JONLNBIO, Jiangsu, China, Cat# JL20633, RRID: AB_3661983); mouse Urea Nitrogen (BUN) ELISA Kit (mouse, Sbjbio, Nanjing, China, Cat# JBS-M0549, RRID: AB_3661991); mouse SOD ELISA KIT (mouse, YBIO, Shanghai, China, Cat# IC-SOD1-Mu, RRID: AB_3661984); mouse IL-18 ELISA KIT (mouse, Chuan Qiu Biotechnology, Shanghai, China, Cat# HM-022, RRID: AB_3661985); mouse IL-1 ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1516, RRID: AB_3661986); tumor necrosis factor-α (TNF-α) ELISA KIT (mouse, Bioss, Beijing, China, Cat# bsk12002, RRID: AB_3661987); mouse MDA ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1331, RRID: AB_3661988); mouse ROS ELISA KIT (mouse, Yan Yu Medical Reagent, Shanghai, China, Cat# YYu-ELISA-374950, RRID: AB_3661989); mouse beta galactosidases (β-GAL) ELISA KIT (mouse, KlBio, Shanghai, China, Cat# kl-M1264c, RRID: AB_3661990).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of ethnopharmacology

Article Title: Anti-fatigue effect of an enzymatically derived deer velvet extract through muscle damage recovery and improvement of antioxidant levels.

doi: 10.1016/j.jep.2024.118965

Figure Lengend Snippet: Fig. 4. Effect of YC-1101 on the expression of fatigue improvement-related biomarkers in serum after forced swimming test. Levels of (A) lactate (B) LDH (C) SOD (D) GPx, and (E) MDA. (F) Schematic representation of the anti-fatigue mechanism of action of YC-1101. LDH: lactate dehydrogenase. SOD: superoxide dis- mutase. GPx: glutathione peroxidase. MDA: malondialdehyde. ARE: antioxidant response elements. Values are expressed as the mean ± standard error of the mean. *p < 0.05, **p < 0.01 vs. normal control. #p < 0.05 YC-1101 vs. YHC-BE-2038.

Article Snippet: Concurrently, blood samples were collected, and serum was obtained by centrifugation at 3000 rpm for 10 min. Serum levels of lactate, LDH, superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) were determined using a lactate assay kit (K627-100, Biovision, San Francisco, CA, USA), LDH assay kit (ab102526, Abcam), SOD assay kit (CSB- E08556m, CUSABIO, Wuhan, China), GPx assay kit (CSBE13068m, CUSABIO), and MDA assay kit (ab118970, Abcam), respectively.

Techniques: Expressing, Control